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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene is observed in several tumors, which leads to chromosome instability, and therefore might add to tumorigenesis. This protein was discovered to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a significant function in advertising and marketing cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have already been described. Two more splice variants have been reported but specific nucleotide sequence details is not nonetheless out there.
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Blocking peptides are peptides that bind specifically for the concentrate on antibody and block antibody binding. These peptide typically incorporates the epitope regarded with the antibody.
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This guide outlines further more doable optimization of cellular and immunoassay parameters to make sure the best possible success are...
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This guidebook outlines more attainable optimization of cellular and immunoassay parameters to ensure the very best success are...
Antibodies sure to the blocking peptide now not bind to your epitope over the concentrate on protein. By evaluating the staining through the blocked antibody as opposed to the antibody on your own, one can see which staining is specific.
Target Information and facts Cyclin E belongs check here into the really conserved cyclin relatives, whose customers are characterised by a spectacular periodicity in protein abundance from the mobile cycle. Cyclins operate as regulators of CDK kinases. Various cyclins show unique expression and degradation designs which contribute to your temporal coordination of every mitotic occasion. Cyclin E sorts a fancy with and functions as a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary and is also degraded as cells development by way of S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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